Foundation Publications

2019

  • [DOI] F. Gasperoni, P. Turini, and E. Agostinelli, “A novel comprehensive paradigm for the etiopathogenesis of multiple sclerosis: therapeutic approaches and future perspectives on its treatment,” Amino acids, vol. 51, iss. 5, p. 745–759, 2019.
    [Bibtex]
    @Article{Gasperoni2019,
    author="Gasperoni, Francesco
    and Turini, Paola
    and Agostinelli, Enzo",
    title="A novel comprehensive paradigm for the etiopathogenesis of multiple sclerosis: therapeutic approaches and future perspectives on its treatment",
    journal="Amino Acids",
    year="2019",
    month="May",
    day="01",
    volume="51",
    number="5",
    pages="745--759",
    abstract="It is well recognized that variation in the geographical distribution of prevalence of multiple sclerosis (MS) exists: increasing the latitude its prevalence increases as well, but the underlying causes of such dissimilarity still remained elusive as of today. Currently, the most accredited hypothesis is that the closer to the equator the more pronounced is the amount of sunlight which, in turn, increases the production of vitamin D. Cholecalciferol is indeed deficient in MS patients, but this factor does not explain by itself the etiopathogenesis of the disease. In the present study, to search for a pattern and provide a model of the disease's etiology consistent with this regional factor, as well with its changing ethnic, sex-ratio, lifestyle variations and the other unexplained aspects of MS, an extensive analysis of peer-reviewed literature and data was conducted. The arisen hypothesis was that, increasing the latitude, the factor that varies and can have the stronger effect on the human organism, is the continuous and ever-increasing diversity of the natural light--dark cycle. The consequent effort of the suprachiasmatic nucleus to entrain the organism's circadian rhythm affects the hypothalamic--pituitary--adrenal axis resulting in desynchronizing the central and peripheral circadian clocks and pathologizing the immunitary system. To verify such hypothesis, a theoretical framework of the etiopathogenesis, coherent with the gathered literature, was conceived and a demonstration to corroborate it was eventually devised and performed. The results underscored that people living in countries subjected to a further circadian disruptive factor, as daylight saving time, have a 6.35 times higher prevalence of MS than States placed on their same latitude that do not observe it, thus strongly supporting the hypothesis. As further reinforcement of the conclusions, it is worth mentioning that the levels of polyamines rise abruptly in autoimmune diseases. Moreover, among their numerous roles, these polycations participate to the regulation of the circadian clock so their sudden variation might disrupt it. Following these interesting findings, new perspectives in therapies are, therefore, proposed.",
    issn="1438-2199",
    doi="10.1007/s00726-019-02718-1",
    url="https://doi.org/10.1007/s00726-019-02718-1"
    }
  • [DOI] S. Coni, L. Di Magno, S. M. Serrao, Y. Kanamori, E. Agostinelli, and G. Canettieri, “Polyamine metabolism as a therapeutic target in hedgehog-driven basal cell carcinoma and medulloblastoma,” Cells, vol. 8, iss. 2, 2019.
    [Bibtex]
    @Article{cells8020150,
    AUTHOR = {Coni, Sonia and Di Magno, Laura and Serrao, Silvia Maria and Kanamori, Yuta and Agostinelli, Enzo and Canettieri, Gianluca},
    TITLE = {Polyamine Metabolism as a Therapeutic Target in Hedgehog-Driven Basal Cell Carcinoma and Medulloblastoma},
    JOURNAL = {Cells},
    VOLUME = {8},
    YEAR = {2019},
    NUMBER = {2},
    ARTICLE-NUMBER = {150},
    URL = {https://www.mdpi.com/2073-4409/8/2/150},
    PubMedID = {30754726},
    ISSN = {2073-4409},
    ABSTRACT = {Hedgehog (Hh) signaling is a critical developmental regulator and its aberrant activation, due to somatic or germline mutations of genes encoding pathway components, causes Basal Cell Carcinoma (BCC) and medulloblastoma (MB). A growing effort has been devoted at the identification of druggable vulnerabilities of the Hedgehog signaling, leading to the identification of various compounds with variable efficacy and/or safety. Emerging evidence shows that an aberrant polyamine metabolism is a hallmark of Hh-dependent tumors and that its pharmacological inhibition elicits relevant therapeutic effects in clinical or preclinical models of BCC and MB. We discuss here the current knowledge of polyamine metabolism, its role in cancer and the available targeting strategies. We review the literature about the connection between polyamines and the Hedgehog signaling, and the potential therapeutic benefit of targeting polyamine metabolism in two malignancies where Hh pathways play a well-established role: BCC and MB.},
    DOI = {10.3390/cells8020150}
    }
  • [DOI] M. L. Di Paolo, M. Cervelli, P. Mariottini, A. Leonetti, F. Polticelli, M. Rosini, A. Milelli, F. Basagni, R. Venerando, E. Agostinelli, and A. Minarini, “Exploring the activity of polyamine analogues on polyamine and spermine oxidase: methoctramine, a potent and selective inhibitor of polyamine oxidase,” Journal of enzyme inhibition and medicinal chemistry, vol. 34, pp. 740-752, 2019.
    [Bibtex]
    @article{article,
    author = {Di Paolo, Maria Luisa and Cervelli, Manuela and Mariottini, Paolo and Leonetti, Alessia and Polticelli, Fabio and Rosini, Michela and Milelli, Andrea and Basagni, Filippo and Venerando, Rina and Agostinelli, Enzo and Minarini, Anna},
    year = {2019},
    month = {03},
    pages = {740-752},
    title = {Exploring the activity of polyamine analogues on polyamine and spermine oxidase: methoctramine, a potent and selective inhibitor of polyamine oxidase},
    volume = {34},
    journal = {Journal of Enzyme Inhibition and Medicinal Chemistry},
    doi = {10.1080/14756366.2019.1584620}
    }
  • [DOI] S. Ohkubo, R. Mancinelli, S. Miglietta, A. Cona, R. Angelini, G. Canettieri, D. A Spandidos, E. Gaudio, and E. Agostinelli, “Maize polyamine oxidase in the presence of spermine/spermidine induces the apoptosis of lovo human colon adenocarcinoma cells,” International journal of oncology, vol. 54, 2019.
    [Bibtex]
    @article{article,
    author = {Ohkubo, Shinji and Mancinelli, Romina and Miglietta, Selenia and Cona, Alessandra and Angelini, Riccardo and Canettieri, Gianluca and A Spandidos, Demetrios and Gaudio, Eugenio and Agostinelli, Enzo},
    year = {2019},
    month = {04},
    pages = {},
    title = {Maize polyamine oxidase in the presence of spermine/spermidine induces the apoptosis of LoVo human colon adenocarcinoma cells},
    volume = {54},
    journal = {International Journal of Oncology},
    doi = {10.3892/ijo.2019.4780}
    }
  • [DOI] M. Magro, D. Baratella, G. Miotto, J. Frömmel, M. Šebela, M. Kopečná, E. Agostinelli, and F. Vianello, “Enzyme self-assembly on naked iron oxide nanoparticles for aminoaldehyde biosensing,” Amino acids, vol. 51, iss. 4, p. 679–690, 2019.
    [Bibtex]
    @Article{Magro2019,
    author="Magro, Massimiliano
    and Baratella, Davide
    and Miotto, Giovanni
    and Fr{\"o}mmel, Jan
    and {\v{S}}ebela, Marek
    and Kope{\v{c}}n{\'a}, Martina
    and Agostinelli, Enzo
    and Vianello, Fabio",
    title="Enzyme self-assembly on naked iron oxide nanoparticles for aminoaldehyde biosensing",
    journal="Amino Acids",
    year="2019",
    month="Apr",
    day="01",
    volume="51",
    number="4",
    pages="679--690",
    abstract="The preservation of enzymatic activity is a fundamental requirement for exploiting hybrid nano-bio-conjugates, and the control over protein--nanoparticle interactions, leading to stable and catalytically active hybrids, represents the key for designing new biosensing platforms. In this scenario, surface active maghemite nanoparticles (SAMNs) represent a new class of naked magnetic nanoparticles, displaying peculiar electrocatalytic features and the ability to selectively bind proteins. Recombinant aminoaldehyde dehydrogenase from tomato (SlAMADH1) was used as a model protein, and successfully immobilized by self-assembly on the surface of naked SAMNs, where its enzymatic activity resulted preserved for more than 6 months. The hybrid nanomaterial (SAMN@SlAMADH1) was characterized by UV--Vis spectroscopy, mass spectrometry, and TEM microscopy, and applied for the development of a biosensor for the determination of aminoaldehydes in alcoholic beverages. Measurements were carried out in a low volume electrochemical flow cell comprising a SAMN modified carbon paste electrode for the coulometric determination of the NADH produced during the enzymatic catalysis. The present findings, besides representing the first example of an electrochemical biosensor for aminoaldehydes in an alcoholic matrix, open the door to the use of immobilized enzymes on naked metal oxides nanomaterials for biosensing.",
    issn="1438-2199",
    doi="10.1007/s00726-019-02704-7",
    url="https://doi.org/10.1007/s00726-019-02704-7"
    }